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Title: Epitope mapping by cysteine mutagenesis: identification of residues involved in recognition by three monoclonal antibodies directed against LamB glycoporin in the outer membrane of Escherichia coli.
identifier: 1485555
description:
epitope description:T336, D379, G382, D385, N387, N389, K392, F398
antigen name:Maltoporin
host organism:Mus musculus BALB/c
antibody name:302
aggregation:
instance of dataset
availability:
available
primaryPublications: 7521310
authorizations:
registration not required
accessURL: http://www.iedb.org/reference/1010671
landingPage: http://www.iedb.org/assay/1485555
type:
Literature
publicationVenue:
FEMS Microbiol Lett
dates:
1994
study type: b cell assays
subject species: Escherichia coli
fullName:
L Notley
C Hillier
T Ferenci
method:
western blot
name:
Epitope mapping by cysteine mutagenesis: identification of residues involved in recognition by three monoclonal antibodies directed against LamB glycoporin in the outer membrane of Escherichia coli.
description:
Substitution of each of the epitope residues with cysteine either reduced or abolished binding of the mAb to bacteria in a dot-blot assay.

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