Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1002507 |
| landingPage: |
http://www.iedb.org/assay/1483927 |
| type: |
Literature
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| publicationVenue: |
Proc Natl Acad Sci U S A
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| dates: |
2006
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| study type: | b cell assays |
| subject species: |
| fullName: |
Mitsugu Akagawa
Sohei Ito
Kazuyo Toyoda
Yoshihisa Ishii
Emi Tatsuda
Takahiro Shibata
Satoru Yamaguchi
Yoshichika Kawai
Kousuke Ishino
Yusuke Kishi
Takahiro Adachi
Takeshi Tsubata
Yoshinari Takasaki
Nobutaka Hattori
Tsukasa Matsuda
Koji Uchida
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| method: |
ELISA
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| name: |
Bispecific abs against modified protein and DNA with oxidized lipids.
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| description: |
Since mAb R310 shares sequence homology with anti-DNA antibodies, the reactivity towards DNA was investigated. It was found that the antibody bound calf thymus ds-DNA but not synthetic oligonucletides, and therefore a natural modification of DNA was suspected as the cross-reacting epitope. By analyzing different aldehyde analogs of the eliciting immunogen as modification of DNA, it was found that recognition was extremely enhanced with 4-oxo-2-nonenal (ONE) as the modification. Different deoxy-monoribonucleotides (G,C, and A) were tested as adducts with ONE, and all were highly reactive with the mAb in ELISA, while the non-modified nucleotides were not recognized.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
