Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1006061 |
| landingPage: |
http://www.iedb.org/assay/1484220 |
| type: |
Literature
|
| publicationVenue: |
Chem Biol
|
| dates: |
2006
|
| study type: | b cell assays |
| subject species: |
| fullName: |
Judit Villé
n
Ricard A Rodrí
guez-Mias
José
I Nú
ñ
ez
Ernest Giralt
Francisco Sobrino
David Andreu
|
| method: |
in vivo assay
|
| name: |
Rational dissection of binding surfaces for mimicking of discontinuous antigenic sites.
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| description: |
The epitope is a construct of sequences from FMDV that form loop structures and comprise an antigenic site, including VP1 (193-195, TGD), VP3 (K84), VP3 (58-60, ENV), VP3 (Y63), VP2 (72-80,PSQNFGHMHK), and a VP2 analog (133-137, SEKDR).
One of 4 guinea pigs immunized with the cyclic epitope was protected from from challenge with FMDV and developed only a single minute lesion on a paw. One of 3 guinea pigs immunized with the cyclic epitope with an insertion of a 6-aminohexanoic acid residue instead of Gly next to the C-terminal Cys was completely protected and a second animal had only minor lesions. Guinea pigs immunized with the cyclic epitope modified by insertion of a 6-aminohexanoic acid or beta-Ala residue next to the N-terminal Cys, were not protected.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
