Rational dissection of binding surfaces for mimicking of discontinuous antigenic sites. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Rational dissection of binding surfaces for mimicking of discontinuous antigenic sites.

Dataset

identifier:	1484198
description:	epitope description:CTGDKENVYPSQNFGHMHKSEKDRGC host organism:Mus musculus antibody name:2A12, 2E5,3E9, 5C4
aggregation:	instance of dataset
availability:	available
primaryPublications:	16931331

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1006061
landingPage:	http://www.iedb.org/assay/1484198

Publication

type:	Literature
publicationVenue:	Chem Biol
dates:	2006

Dimension

study type:	b cell assays
subject species:

Person

fullName:	Judit Villé n Ricard A Rodrí guez-Mias José I Nú ñ ez Ernest Giralt Francisco Sobrino David Andreu

Data Type

method:	ELISA

Study

name:	Rational dissection of binding surfaces for mimicking of discontinuous antigenic sites.
description:	The epitope is a construct of sequences from FMDV that form loop structures and comprise an antigenic site, including VP1 (193-195, TGD), VP3 (K84), VP3 (58-60, ENV), VP3 (Y63), VP2 (72-80,PSQNFGHMHK), and a VP2 analog (133-137, SEKDR). MAbs 2A12, 2E5,3E9, and 5C4, which recognize conformational epitopes, reacted with the linear epitope or the cyclic peptide, oxidized to form an intrachain disulfide bond. Insertion of a 6-aminohexanoic acid residue instead of Gly next to the C-terminal Cys improved antibody recognition, especially of the cyclic peptide.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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