Monoclonal antibody epitope mapping describes tailspike beta-helix folding and aggregation intermediates.

Title:

Monoclonal antibody epitope mapping describes tailspike beta-helix folding and aggregation intermediates.

identifier:	1487473
description:	epitope description:K164, W203, D239, G245, V332 antigen name:Tail fiber protein host organism:Mus musculus BALB/c antibody name:Abs 33, 175 and 236
aggregation:	instance of dataset
availability:	available
primaryPublications:	15833745

study type:	b cell assays
subject species:	Salmonella virus P22

fullName:	Madhulika Jain Michael S Evans Jonathan King Patricia L Clark

method:	ELISA

name:	Monoclonal antibody epitope mapping describes tailspike beta-helix folding and aggregation intermediates.
description:	Tailspike-specific mAbs were used against tailspike mutants to map epitopes on the wild-type protein. The disruption of binding following mutations at these residues suggested that they play a role in binding on the native protein. Kd values for mAbs 33, 175 and 236 were , 3nM, 0.1nM and 0.1nM, respectively. These mAbs preferentially recognizes native conformation.

name:	iedb
homePage:	http://www.iedb.org

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