The last two cytoplasmic loops in the lactose permease of Escherichia coli comprise a discontinuous epitope for a monoclonal antibody.

Title:

The last two cytoplasmic loops in the lactose permease of Escherichia coli comprise a discontinuous epitope for a monoclonal antibody.

identifier:	1487535
description:	epitope description:I283, K289, F334, K335, E342, V343, R344, F345 antigen name:Lactose permease host organism:Mus musculus BALB/c antibody name:4B11
aggregation:	instance of dataset
availability:	available
primaryPublications:	8993344

study type:	b cell assays
subject species:	Escherichia coli

fullName:	J Sun J Li N Carrasco H R Kaback

method:	surface plasmon resonance (SPR)

name:	The last two cytoplasmic loops in the lactose permease of Escherichia coli comprise a discontinuous epitope for a monoclonal antibody.
description:	Epitope-specific mAb(s) did not bind lactose permease in dot blot. Single Cys replacement mutants E342C and R344C abolished mAb binding, establishing these two residues as primary determinant. Cysteine substitution of other epitopic residues (I283, K289, F334, K335, V343, and F345) resulted in reduced mAb binding.

name:	iedb
homePage:	http://www.iedb.org

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