Epitope mapping of the cat (Felis domesticus) major allergen Fel d I by overlapping synthetic peptides and monoclonal antibodies against native and denatured Fel d I.

Title:

Epitope mapping of the cat (Felis domesticus) major allergen Fel d I by overlapping synthetic peptides and monoclonal antibodies against native and denatured Fel d I.

Dataset

identifier:	1488270
description:	epitope description:LLDKIYTSPLC antigen name:Other Felis catus (cat) protein host organism:Mus musculus BALB/c antibody name:FdR-1a, C5/24.6
aggregation:	instance of dataset
availability:	available
primaryPublications:	7687098

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1005323
landingPage:	http://www.iedb.org/assay/1488270

Publication

type:	Literature
publicationVenue:	Allergy
dates:	1993

Dimension

study type:	b cell assays
subject species:	Felis catus

Person

fullName:	W van 't Hof F J van Milligen M van den Berg M Lombardero M D Chapman R C Aalberse

Data Type

method:	radio immuno assay (RIA)

Study

name:	Epitope mapping of the cat (Felis domesticus) major allergen Fel d I by overlapping synthetic peptides and monoclonal antibodies against native and denatured Fel d I.
description:	MAb FdR-1a, which recognized the epitope and the overlapping peptide (residues 53-66), bound to reduced and alkylated Fel d 1 better than to native Fel d 1, as shown by their ability to inhibit the binding of mAb FdR-1a to the epitope. MAb C5/24.6, which recognized the epitope and other peptides from chain 1 and chain 2, bound to native Fel d 1, as shown by its ability to inhibit the binding of mAb C5/24.6 to the epitope

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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