Antibody recognition of a flexible epitope at the DNA binding site of the human papillomavirus transcriptional regulator E2.

Title:

Antibody recognition of a flexible epitope at the DNA binding site of the human papillomavirus transcriptional regulator E2.

identifier:	1492013
description:	epitope description:N296, Y303, K327 antigen name:Regulatory protein E2 host organism:Mus musculus BALB/c antibody name:ED15
aggregation:	instance of dataset
availability:	available
primaryPublications:	17176073

study type:	b cell assays
subject species:	Human papillomavirus type 16

fullName:	Marí a Laura Cerutti Diego U Ferreiro Santiago Sanguineti Fernando A Goldbaum Gonzalo de Prat-Gay

method:	surface plasmon resonance (SPR)

name:	Antibody recognition of a flexible epitope at the DNA binding site of the human papillomavirus transcriptional regulator E2.
description:	These residues are located on the DNA binding helix of the C terminal region of the protein. This epitope-specific mAb affinity to the E2C domain was measured. The effect of ionic strength and pH on the kinetic constants was studied by SPR and calorimetry measurements. The interaction was determined to be highly influenced by changes in ionic strength and to be entropically driven.

name:	iedb
homePage:	http://www.iedb.org

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