Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1006627 |
| landingPage: |
http://www.iedb.org/assay/1493915 |
| type: |
Literature
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| publicationVenue: |
Infect Immun
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| dates: |
1996
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| study type: | b cell assays |
| subject species: | Treponema pallidum subsp. pallidum str. Nichols |
| fullName: |
R E Baughn
M Demecs
L H Taber
D M Musher
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| method: |
ELISA
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| name: |
Epitope mapping of B-cell determinants on the 15-kilodalton lipoprotein of Treponema pallidum (Tpp15) with synthetic peptides.
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| description: |
IgM fractions from 6 infants with symptomatic congenital syphilis were tested with overlapping 8-mers to define the regions of interest. Further definition of this epitope with shorter peptides was subsequently done and this 7-mer was the optimal peptide. Alanine replacement of each residue showed that residues Phe at position 4 and Glu at position 6 were essential for antibody binding, while the alanine replacement of K2 residue enhanced binding.
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| name: |
iedb
|
| homePage: |
http://www.iedb.org |
