Immunological Data Discovery Index
Immunological Data Discovery Index
| Title: | The identification and characterization of a monoclonal antibody to the vaccinia virus E3 protein. |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1007678 |
| landingPage: |
http://www.iedb.org/assay/1493378 |
| type: |
Literature
|
| publicationVenue: |
Virus Res
|
| dates: |
2007
|
| study type: | b cell assays |
| subject species: | Vaccinia virus WR |
| fullName: |
Jessica R Weaver
Mohammad Shamim
Edward Alexander
D Huw Davies
Philip L Felgner
Stuart N Isaacs
|
| method: |
western blot
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| name: |
The identification and characterization of a monoclonal antibody to the vaccinia virus E3 protein.
|
| description: |
The epitope is derived from the protein sequence reported by Chang and Jacobs Virology. 1993 Jun
194(2):537-47. [PMID: 8099244].
The epitopic region was mapped using two Vaccinia virus WR C-terminal deletion mutants. The epitope-specific monoclonal antibody was also shown to cross-react to several orthopoxviruses, including several vaccinia virus strains (Dryvax, Lister, IHDJ), rabbitpox virus, cowpox virus, and ectromelia virus. The epitopic region in cowpox virus (Brighton) contains a single amino acid difference when compared to vaccinia virus strain WR at residue 181 (lysine to threonine). The authors suggest the low reactivity may mean that this amino acid is important for antibody binding.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |