| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1010650 |
| landingPage: |
http://www.iedb.org/assay/1494408 |
| type: |
Literature
|
| publicationVenue: |
Immunol Lett
|
| dates: |
2001
|
| study type: | b cell assays |
| subject species: | Neisseria meningitidis serogroup B CU385 |
| fullName: |
T Mené
ndez
I De Haz
M Delgado
H Garay
A Martí
n
N S Vispo
|
| method: |
ELISA
|
| name: |
Immunisation with phage-displayed variable region 2 from meningococcal PorA outer membrane protein induces bactericidal antibodies against Neisseria meningitidis.
|
| description: |
The epitope was synthesized with Cys residues at the N- and C-termini.
Sera from mice immunized with phage, displaying the epitope with Cys residues added to the N-and C-termini, recognized PorA OMP in outer membrane vesicles by ELISA and Western blotting. Sera from mice immunized with phage, displaying the epitope synthesized with Cys and 3 Gly residues added to the N-and C-termini, did not significantly recognize PorA OMP by ELISA and Western blotting.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |