Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 1495509 |
| description: |
epitope description:K385, E386, C390, P391, C392, E393, P394, E395, N408, C409, V410
antigen name:Apical merozoite antigen 1
host organism:Mus musculus BALB/c
antibody name:F8.12.19
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| aggregation: |
instance of dataset
|
| availability: |
available
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| primaryPublications: |
17229439 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1012916 |
| landingPage: |
http://www.iedb.org/assay/1495509 |
| type: |
Literature
|
| publicationVenue: |
J Mol Biol
|
| dates: |
2007
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| study type: | b cell assays |
| subject species: | Plasmodium vivax |
| fullName: |
Sé
bastien Igonet
Brigitte Vulliez-Le Normand
Grazyna Faure
Marie-Madeleine Riottot
Clemens H M Kocken
Alan W Thomas
Graham A Bentley
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| method: |
x-ray crystallography
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| name: |
Cross-reactivity studies of an anti-Plasmodium vivax apical membrane antigen 1 monoclonal antibody: binding and structural characterisation.
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| description: |
The Gene Bank epitope residue positions correspond to the alignment of PDB and Gene Bank sequences provided at the PDB web-site.
The epitope of F8.12.19 on the P. vivax AMA1 protein was determined from the crystal structure of the complex. Only the 34-residue segment (Ile421 to Lys454) of the complete PvAMA1 ectoplasmic region could be identified in the electron density. Superposition of the complete structure of the PvAMA1 ectoplasmic region [PDB:1W8K] onto the region seen in the electron density shows that the intact antigen cannot be accommodated in the crystal lattice of the structure, suggesting that parts of the antigen were removed by proteolysis prior to crystal formation. No additional residues of the cognate antigen could be within contact distance from F8.12.19, suggesting that this epitope is a complete epitope recognized by the Fab on PvAMA1.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
