Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1007652 |
| landingPage: |
http://www.iedb.org/assay/1495552 |
| type: |
Literature
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| publicationVenue: |
Mol Immunol
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| dates: |
2008
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| study type: | b cell assays |
| subject species: | Influenza A virus (A/X-47(H3N2)) |
| fullName: |
Sabrina Deroo
Auré
lie Fischer
Nadia Beaupain
Manuel Counson
Nathalie Boutonnet
Jurgen Pletinckx
Stefan Loverix
Els Beirnaert
Hans De Haard
Jean-Claude Schmit
Ignace Lasters
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| method: |
surface plasmon resonance (SPR)
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| name: |
Non-immunized natural human heavy chain CDR3 repertoires allow the isolation of high affinity peptides mimicking a human influenza hemagglutinin epitope.
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| description: |
The source cited in the original paper (Wilson et al, Cell. 1984 37(3):767 [PMID 6204768]) for this peptide is the hybrid Influenza A X-47 HA, but no entries corresponding to that strain HA sequence could be found in the current genebank/SP databases. The accession chosen is for the source of the HA molecule in the hybrid virus, and contains the epitope and the peptide used for the immunization procedures, as stated in the cited reference.
The affinity of the anti-peptide mAb for the epitope was measured in ELISA and SPR. The KD values obtained were 5.5 and 9 nM, respectively.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
