Non-immunized natural human heavy chain CDR3 repertoires allow the isolation of high affinity peptides mimicking a human influenza hemagglutinin epitope.

Title:

Non-immunized natural human heavy chain CDR3 repertoires allow the isolation of high affinity peptides mimicking a human influenza hemagglutinin epitope.

Dataset

identifier:	1495563
description:	epitope description:SPAPERRGYSGYDVPDY antigen name:Other Homo sapiens (human) protein host organism:Mus musculus antibody name:17/9
aggregation:	instance of dataset
availability:	available
primaryPublications:	17936360

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1007652
landingPage:	http://www.iedb.org/assay/1495563

Publication

type:	Literature
publicationVenue:	Mol Immunol
dates:	2008

Dimension

study type:	b cell assays
subject species:	Homo sapiens

Person

fullName:	Sabrina Deroo Auré lie Fischer Nadia Beaupain Manuel Counson Nathalie Boutonnet Jurgen Pletinckx Stefan Loverix Els Beirnaert Hans De Haard Jean-Claude Schmit Ignace Lasters

Data Type

method:	surface plasmon resonance (SPR)

Study

name:	Non-immunized natural human heavy chain CDR3 repertoires allow the isolation of high affinity peptides mimicking a human influenza hemagglutinin epitope.
description:	The epitope sequence corresponds to the CDR3 loop of the heavy chain of a non-immunized human IgM antibody. It was shown to mimic the HA1 peptide from Influenza A in its binding to a mouse mAb. Affinity of the anti-HA mAb to the epitope was measured by ELISA and SPR, and the KD values obtained were 7.6 and 92 nM, respectively.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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