Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1009032 |
| landingPage: |
http://www.iedb.org/assay/1495969 |
| type: |
Literature
|
| publicationVenue: |
Proc Natl Acad Sci U S A
|
| dates: |
1991
|
| study type: | b cell assays |
| subject species: | Homo sapiens |
| fullName: |
A McClelland
J deBear
S C Yost
A M Meyer
C W Marlor
J M Greve
|
| method: |
flow cytometry
|
| name: |
Identification of monoclonal antibody epitopes and critical residues for rhinovirus binding in domain 1 of intercellular adhesion molecule 1.
|
| description: |
MAB c78.5A recognizes a conformation-dependent epitope in domain 1 (residues 1-88) of human ICAM-1 as shown by binding to human-mouse chimeric molecules consisting of human domain 1 and the remainder of mouse ICAM-1. The critical residues for antibody recognition were shown to be P70, D71, and G72. Binding of mAb c78.5A to ICAM-1 was reduced by the single mutation D71G and the double mutation replacing residues 71 and 72 (DG) with TV, and abolished by the triple mutation replacing residues 70-72 (PDG) with GTV. These substitutions also reduced binding of human rhinovirus 3 to cells expressing the mutant ICAM-1.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
