Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1012181 |
| landingPage: |
http://www.iedb.org/assay/1499154 |
| type: |
Literature
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| publicationVenue: |
Intervirology
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| dates: |
2002
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| study type: | b cell assays |
| subject species: | Plasmodium falciparum |
| fullName: |
Matti Sä
llberg
Janice Hughes
Joyce Jones
Tom R Phillips
David R Milich
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| method: |
ELISA
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| name: |
A malaria vaccine candidate based on a hepatitis B virus core platform.
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| description: |
Mice immunized with the HBcAg-CSP hybrid containing the NANP, NVDP and CSP 326-345 epitopes responded with high antibody titer to the epitope. Separate assays demonstrated that the NVDP epitope significantly enhanced titers (2-3 orders of magnitude) compared to NANP alone or NANP plus CSP (326-345). The C-terminal Cys (C342) of the CSP (326-345) epitope is critical for the stability and therefore immunogenicity of the immunogen complex. High titers were achieved by week 2, plateaued at week 10. Boosting at week 10 showed a 2-fold increase. IgG2b and IgG3 predominated early in the response, followed by IgG1 and IgG2a later. Of the adjuvants tested, only Montanide, MPL-SE and RAS performed nearly as well as CFA/IFA. H-2 congenic mice were used to confirm the lack of non-responders to NANP using this formulation.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
