Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1013132 |
| landingPage: |
http://www.iedb.org/assay/1500046 |
| type: |
Literature
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| publicationVenue: |
Mol Immunol
|
| dates: |
2008
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| study type: | b cell assays |
| subject species: |
| fullName: |
Luis F Pacios
Leticia Tordesillas
Javier Cuesta-Herranz
Esther Compes
Rosa Sá
nchez-Monge
Arantxa Palací
n
Gabriel Salcedo
Araceli Dí
az-Perales
|
| method: |
ELISA
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| name: |
Mimotope mapping as a complementary strategy to define allergen IgE-epitopes: peach Pru p 3 allergen as a model.
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| description: |
Sequences such as this peptide were obtained by panning a synthetic library with anti-Pru p 3 allergen antisera. They conform to the consensus sequence TSRPALLNDQGH, which was mapped onto the Pru p 3 molecule, by selecting residues located at the protein surface that exhibit proper electrostatic and solvent exposure features and choosing analogous amino acids to replace those absent in Pru p 3, to the residues N35, N36, L37, R39, T40, D43, A46, S76, I77 and P78.
A purified Pru p 3-specific IgE fraction was obtaiened by affinity selection from a pool of sera. It was used to screen a random display peptide phage library to select IgE-binding phage clones. The reactivity to this selected clone was confirmed in a phage ELISA.
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| name: |
iedb
|
| homePage: |
http://www.iedb.org |
