Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1006379 |
| landingPage: |
http://www.iedb.org/assay/1503050 |
| type: |
Literature
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| publicationVenue: |
Arch Virol
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| dates: |
2001
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| study type: | b cell assays |
| subject species: | Porcine circovirus 2 |
| fullName: |
C
D Mahe
P Blanchard
M Le Dimna
F Madec
A Jestin
E Albina
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| method: |
ELISA
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| name: |
Identification of an immunorelevant ORF2 epitope from porcine circovirus type 2 as a serological marker for experimental and natural infection.
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| description: |
This epitope sequence is from the reference cited: Mahe et al. (2000) J Gen Virol 81: 1815-1824 [PMID: 10859388].
Sera from PCV2-immunized specific pathogen-free piglets or conventional farm piglets reacted with the epitope in a pepscan analysis of overlapping peptides. Reactivity with the epitope by ELISA increased greater than 15-fold in the SPF piglets and 21-fold in the farm piglets 6 weeks post-inoculation compared to the pre-immune serum. The ratio of reactivity with the epitope versus the analogous peptide from PCV1 was 5.7 and 11.3 in the SPF and farm piglets, respectively, indicating the epitope is PCV2 discriminant. The results of 4 trials showed that 7 of 32 SPF piglets and 16 of 24 farm piglets had antibodies to the epotope by 6 weeks post-inoculation and 7 of 7 SPF piglets seroconverted by 11 weeks.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
