Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1006379 |
| landingPage: |
http://www.iedb.org/assay/1503051 |
| type: |
Literature
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| publicationVenue: |
Arch Virol
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| dates: |
2001
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| study type: | b cell assays |
| subject species: | Porcine circovirus 2 |
| fullName: |
C
D Mahe
P Blanchard
M Le Dimna
F Madec
A Jestin
E Albina
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| method: |
ELISA
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| name: |
Identification of an immunorelevant ORF2 epitope from porcine circovirus type 2 as a serological marker for experimental and natural infection.
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| description: |
This epitope sequence is from the reference cited: Mahe et al. (2000) J Gen Virol 81: 1815-1824 [PMID: 10859388]. An internal source ID is assigned, because the sequence provided in the reference differs from those found by BLAST at position 1 (Ser to Phe substitution).
Sera from PCV2-immunized specific pathogen-free piglets or conventional farm piglets reacted with the epitope in a pepscan analysis of overlapping peptides. Reactivity with the epitope by ELISA increased 9-fold in the SPF piglets and >10-fold in the farm piglets 6 weeks post-inoculation compared to the pre-immune serum. The ratio of reactivity with the epitope versus the analogous peptide from PCV1 was 0.3 in the both piglet groups, indicating the epitope is not PCV2 discriminant.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
