Delineation of a linear epitope by multiple peptide synthesis and phage display. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Delineation of a linear epitope by multiple peptide synthesis and phage display.

Dataset

identifier:	1504504
description:	epitope description:GSYNRSSFESSSGLV antigen name:Capsid protein host organism:Mus musculus BALB/c antibody name:57P
aggregation:	instance of dataset
availability:	available
primaryPublications:	11226482

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1010651
landingPage:	http://www.iedb.org/assay/1504504

Publication

type:	Literature
publicationVenue:	J Immunol Methods
dates:	2001

Dimension

study type:	b cell assays
subject species:	Tobacco mosaic virus

Person

fullName:	L Choulier D Laune G Orfanoudakis H Wlad J Janson C Granier D Altschuh

Data Type

method:	western blot

Study

name:	Delineation of a linear epitope by multiple peptide synthesis and phage display.
description:	Fab 57P reacted with the epitope in a spot blot of overlapping 15-mer peptides spanning residues 122-158 of TMVP. The epitope core shared by positive peptides was residues 139-145 (YNRSSFE). Amino acid substitutions indicated that residues N140, R141, and F144 at positions 4, 5, and 8 of the epitope are critical for antibody binding and showed reduced binding with all substitutions. Residue S143 at position 7 was previously shown to be essential for binding.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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