Reduced allergenic potency of VR9-1, a mutant of the major shrimp allergen Pen a 1 (tropomyosin).

Title:

Reduced allergenic potency of VR9-1, a mutant of the major shrimp allergen Pen a 1 (tropomyosin).

identifier:	1505684
description:	epitope description:DEERM antigen name:Pen a 1 allergen host organism:Homo sapiens
aggregation:	instance of dataset
availability:	available
primaryPublications:	16339577

study type:	b cell assays
subject species:	Farfantepenaeus aztecus

fullName:	Gerald Reese Julia Viebranz Susan M Leong-Kee Matthew Plante Iris Lauer Stefanie Randow Mar San-Miguel Moncin Rosalia Ayuso Samuel B Lehrer Stefan Vieths

method:	western blot

name:	Reduced allergenic potency of VR9-1, a mutant of the major shrimp allergen Pen a 1 (tropomyosin).
description:	The epitope sequence recognized by serum IgE from shrimp-allergic subjects was determined in a study of overlapping peptides. The epitope core sequence was DEERM of the peptides (RSLSDEERM, SLSDEERM, LSDEERMDALEN, SDEERMDA, DEERMDALE) recognized by sera from 6 of 10 patients. Critical positions for antibody recognition were determined by analysis of the epitope substituted with amino acids found in the homologous sequence from nonallergenic vertebrate tropomyosins. Amino acid substitution R140K at positions 4 of the epitope sequence abolished IgE reactivity of the 2 of the 6 sera.

name:	iedb
homePage:	http://www.iedb.org

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