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Title: Epitope mapping and accessibility of immunodominant regions of yeast plasma membrane H(+)-ATPase.
identifier: 1542320
description:
epitope description:VDDEDSD
antigen name:Plasma membrane ATPase 1
host organism:Mus musculus
antibody name:6 (AT1-1-B4/1-E9), 18 (AT1-4-C3/1-G12)
aggregation:
instance of dataset
availability:
available
primaryPublications: 7681777
authorizations:
registration not required
accessURL: http://www.iedb.org/reference/1011285
landingPage: http://www.iedb.org/assay/1542320
type:
Literature
publicationVenue:
Eur J Biochem
dates:
1993
study type: b cell assays
subject species: Saccharomyces cerevisiae
fullName:
R Serrano
B C Monk
J M Villalba
C Montesinos
E W Weiler
method:
cross blocking
name:
Epitope mapping and accessibility of immunodominant regions of yeast plasma membrane H(+)-ATPase.
description:
The epitope sequence is from the reference cited: Serrano et al.(1986) Nature 319: 689-693 [PMID: 3005867]. The epitope was deduced.
The epitope-specific mAbs recognized the 100 kDa ATPase from crude yeast membranes by Western blotting. Detergent-purified ATPase competed for the binding of the mAbs to ATPase. Plasma membrane vesicles containing ATPase did not compete for mAb binding, indicating the epitope was not accessible.

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