Epitope analysis using kinetic measurements of antibody binding to synthetic peptides presenting single amino acid substitutions.

Title:

Epitope analysis using kinetic measurements of antibody binding to synthetic peptides presenting single amino acid substitutions.

identifier:	1512821
description:	epitope description:INNLIVELIRGT antigen name:Capsid protein host organism:Mus musculus BALB/c antibody name:a4x5
aggregation:	instance of dataset
availability:	available
primaryPublications:	7508237

study type:	b cell assays
subject species:	Tobacco mosaic virus

fullName:	G Zeder-Lutz D Altschuh S Denery-Papini J P Briand G Tribbick M H Van Regenmortel

method:	surface plasmon resonance (SPR)

name:	Epitope analysis using kinetic measurements of antibody binding to synthetic peptides presenting single amino acid substitutions.
description:	The kinetic constants of peptides with single amino acid substitutions were also determined. The largest difference was unexpectedly noted for substitution Leu to Ser at position 4, which led to a 2-fold increase in the association constant (ka), a 78-fold increase in the dissociation constant (kd), and a 42-fold decrease in the equilibrium affinity constant (Ka). A large difference also was noted for substitution Ile to Leu at position 5, which led to >2-fold increase in the association constant (ka), a 11-fold increase in the dissociation constant (kd), and a 5-fold decrease in the equilibrium affinity constant (Ka). The mAb did not bind to peptides with substitutions of Glu to Gln at position 7 or Ile to Lys at position 9.

name:	iedb
homePage:	http://www.iedb.org

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