Kinetic analysis of the effect on Fab binding of identical substitutions in a peptide and its parent protein.

Title:

Kinetic analysis of the effect on Fab binding of identical substitutions in a peptide and its parent protein.

identifier:	1513049
description:	epitope description:LDPLVTALLGAFD antigen name:Capsid protein host organism:Mus musculus BALB/c antibody name:270P
aggregation:	instance of dataset
availability:	available
primaryPublications:	10090739

study type:	b cell assays
subject species:	Tobacco mosaic virus

fullName:	L Choulier N Rauffer-Bruyè re M Ben Khalifa F Martin D Altschuh

method:	surface plasmon resonance (SPR)

name:	Kinetic analysis of the effect on Fab binding of identical substitutions in a peptide and its parent protein.
description:	The epitope sequence is cited in The epitope sequence is cited in Moudallal et al. (1985) EMBO J. 4:1231-1235 [PMID:16453613]. CPmutHis contains two mutations, T28I and E95D, to reduce aggregation properties of the protein. Binding parameters for the binding of the epitope-specific mAb to the antigen were determined using a BIAcore 2000 instrument. Mutations in the epitope (S142E, S142N, or E145A) did not lead to significant changes in binding. The epitope was synthesized with Lys-Gly added to the N-terminus.

name:	iedb
homePage:	http://www.iedb.org

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