Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1013324 |
| landingPage: |
http://www.iedb.org/assay/1548560 |
| type: |
Literature
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| publicationVenue: |
Anal Chem
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| dates: |
2008
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| study type: | b cell assays |
| subject species: | Influenza A virus (A/Shandong/9/1993(H3N2)) |
| fullName: |
Bethny Morrissey
Kevin M Downard
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| method: |
mass spectrometry
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| name: |
Kinetics of antigen-antibody interactions employing a MALDI mass spectrometry immunoassay.
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| description: |
The sequence is conserved in other H3N2 strains such as Kiev/301/94 and Panama/2007/99. The cysteine at position 5 has a carboxyamidomethyl group as a naturally ocurring post-translational modification. This fragment and the region 158-166 (GSVNSFFSR) are recognized by the same mAb and reside in close proximity atop the HA1 subunit suggesting that they form part of a discontinuous epitope.
Both the tryptic peptide where Cys at position 5 supports the naturally ocurring post-translational modification and a second form in which this modification is replaced with a beta-hydroxyethyl group (as a result of the pretreatment of the antigen with beta-hydroxyethanol ahead of digestion) are detected. The mAb reacted with two tryptic fragments of the Shangdong and Kiev strains (which have the same sequences) as detected in Maldi-TOF spectra analysis. These fragments (109-125 and 158-166) reside in close proximity atop the HA1 subunit suggesting that they form part of a discontinuous epitope.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
