Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1013350 |
| landingPage: |
http://www.iedb.org/assay/1550373 |
| type: |
Literature
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| publicationVenue: |
J Immunol
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| dates: |
2003
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| study type: | b cell assays |
| subject species: | Human T-lymphotropic virus 1 |
| fullName: |
Cyril J Cohen
Offra Sarig
Yoshihisa Yamano
Utano Tomaru
Steven Jacobson
Yoram Reiter
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| method: |
flow cytometry
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| name: |
Direct phenotypic analysis of human MHC class I antigen presentation: visualization, quantitation, and in situ detection of human viral epitopes using peptide-specific, MHC-restricted human recombinant antibodies.
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| description: |
The epitope sequence is from the reference cited: Nagai et al. (2001) J Infect Dis 183: 197-205 [PMID: 11120926].
TCR-like Fab antibodies specific for the HLA-A2/epitope complex were selected from a large human Fab phage library using a single chain construct of HLA-A2 linked to beta2m complexed with the epitope. Fabs T3F2 and T3E3 detected the epitope complexed with HLA-A2 on the surface of APCs pulsed with the epitope, such as murine RMA-S transfected with HLA-A2, HLA-A2+ EBV-transformed B cell line JY, or human HLA-A2+ dendritic cells. Fab tetramers showed 2-log increased staining intensity.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
