Epitope mapping and functional characterization of monoclonal antibodies specific for the alpha subunit of Escherichia coli RNA polymerase.

Title:

Epitope mapping and functional characterization of monoclonal antibodies specific for the alpha subunit of Escherichia coli RNA polymerase.

identifier:	1587796
description:	epitope description:RGLSLGMRLEN antigen name:DNA-directed RNA polymerase subunit alpha host organism:Mus musculus SJL antibody name:130B1, 125C6
aggregation:	instance of dataset
availability:	available
primaryPublications:	7522231

study type:	b cell assays
subject species:	Escherichia coli

fullName:	K A Sharif N Fujita R Jin K Igarashi A Ishihama J S Krakow

method:	immunoprecipitation

name:	Epitope mapping and functional characterization of monoclonal antibodies specific for the alpha subunit of Escherichia coli RNA polymerase.
description:	The sequence source was obtained through cited reference: Igarashi et al. Nucleic Acids Res 1990 18:5945-5948 [PMID 2235479]. The mAbs 130B1 and 125C6 bound both the 210-329 and 210-320 fragments of the RNA polymerase, but not 210-310. The authors therefore deduced that the mAbs were specifically binding 310-320 as the epitope.

name:	iedb
homePage:	http://www.iedb.org

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on Feedback.