Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1013042 |
| landingPage: |
http://www.iedb.org/assay/1584199 |
| type: |
Literature
|
| publicationVenue: |
Mol Immunol
|
| dates: |
1989
|
| study type: | b cell assays |
| subject species: | Foot-and-mouth disease virus (strain A10-61) |
| fullName: |
W M Schaaper
H Lankhof
W C Puijk
R H Meloen
|
| method: |
ELISA
|
| name: |
Manipulation of antipeptide immune response by varying the coupling of the peptide with the carrier protein.
|
| description: |
Antisera from rabbits immunized with the epitope conjugated to KLH via the N- or C-terminus through an added Cys residue reacted with the epitope. Using replacement sets of peptides, in which each residue is substituted by each of 19 other amino acids, it was shown that Pro and Ala at positions 7 and 8 were critical for binding antisera raised to the N-terminally coupled epitope. In contrast, Gln at position 3 was the most important for binding antisera raised to the C-terminally coupled epitope.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
