Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1005292 |
| landingPage: |
http://www.iedb.org/assay/1590061 |
| type: |
Literature
|
| publicationVenue: |
Mol Immunol
|
| dates: |
1995
|
| study type: | b cell assays |
| subject species: | Dermatophagoides farinae |
| fullName: |
C Nishiyama
M Fukada
Y Usui
N Iwamoto
Y Okumura
H Okudaira
|
| method: |
antigen inhibition
|
| name: |
Analysis of the IgE-epitope of Der f 2, a major mite allergen, by in vitro mutagenesis.
|
| description: |
The epitope was deduced.
Truncation of the C-terminal 9 residues residues of Der f 2 significantly reduced binding to patient serum IgE. Truncation of the C-terminal 6 or 7 residues also reduced binding. Truncation constructs of Der f 2 with the C-terminal residues (121-129) deleted showed significantly reduced inhibition of patient serum IgE binding to Der f 2. Single alanine substitution of residues in this region did not dramatically reduce IgE binding activity of the mutant Der f 2. The authors conclude that region 121-129 contains part of an IgE-binding epitope. Disruption of the disulfide bond between Cys8-Cys119 may contribute to the reduced binding of the truncation mutants.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
