Mapping and mutational analysis of the IgE-binding epitopes on Ara h 1, a legume vicilin protein and a major allergen in peanut hypersensitivity.

Title:

Mapping and mutational analysis of the IgE-binding epitopes on Ara h 1, a legume vicilin protein and a major allergen in peanut hypersensitivity.

identifier:	1603157
description:	epitope description:LEYDPRCVYD antigen name:Ara h 1 host organism:Homo sapiens
aggregation:	instance of dataset
availability:	available
primaryPublications:	9151961

study type:	b cell assays
subject species:	Arachis hypogaea

fullName:	A W Burks D Shin G Cockrell J S Stanley R M Helm G A Bannon

method:	western blot

name:	Mapping and mutational analysis of the IgE-binding epitopes on Ara h 1, a legume vicilin protein and a major allergen in peanut hypersensitivity.
description:	The epitope was determined by use of overlapping 15- and 10-mers covering the Ara h 1 sequence. The peptide was tested for recognition of individual patient's serum. With a pool of donors' sera, the reactivity towards single residue substituted peptides was assayed and showed that residues Y67, D68, P69, V72 and Y73 are required for binding, while L65 and E66 substitution diminished binding strength to less than 50% of the WT.

name:	iedb
homePage:	http://www.iedb.org

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