Germline V-genes sculpt the binding site of a family of antibodies neutralizing human cytomegalovirus.

Title:

Germline V-genes sculpt the binding site of a family of antibodies neutralizing human cytomegalovirus.

identifier:	1606360
description:	epitope description:ETIYNTTLKY antigen name:Envelope glycoprotein B host organism:Homo sapiens antibody name:8F9
aggregation:	instance of dataset
availability:	available
primaryPublications:	18772881

study type:	b cell assays
subject species:	Human betaherpesvirus 5

fullName:	Christy A Thomson Steve Bryson Gary R McLean A Louise Creagh Emil F Pai John W Schrader

method:	surface plasmon resonance (SPR)

name:	Germline V-genes sculpt the binding site of a family of antibodies neutralizing human cytomegalovirus.
description:	The epitope-specific, hypermutated mAb affinity for the fragment 64-78 gB was analyzed by SPR. The Fab displayed both fast on and off rates of binding. The off rate was highly dependent on the temperature. Binding was also detected in direct ELISA, and the binding rate was higher at lower tempratures, in accordance with this enhanced disocciation at higher temperature.

name:	iedb
homePage:	http://www.iedb.org

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