Isolation of antibodies specific to a single conformation-dependant antigenic determinant on the EG95 hydatid vaccine.

Title:

Isolation of antibodies specific to a single conformation-dependant antigenic determinant on the EG95 hydatid vaccine.

identifier:	1605461
description:	epitope description:GFGKDITTGHSFTHKSNNDP host organism:Ovis aries
aggregation:	instance of dataset
availability:	available
primaryPublications:	19095030

study type:	b cell assays
subject species:

fullName:	A J Read J L Casey A M Coley M Foley C G Gauci D C Jackson M W Lightowlers

method:	ELISA

name:	Isolation of antibodies specific to a single conformation-dependant antigenic determinant on the EG95 hydatid vaccine.
description:	The identification of epitopes was based on screening two random peptide phage-display libraries with polyclonal antibodies that are specifically directed against the conformation-dependant epitope(s) of the protein. This phage-displayed peptide was competed in the binding to the antibodies by the EG95 protein, by the free synthetic peptide and by the similarly selected peptides E100 (HYKWLNDPLAAW) and E108 (LDKRNNDPFHLP), suggesting they all mimic the same epitope on EG95.

name:	iedb
homePage:	http://www.iedb.org

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