Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 1796308 |
| description: |
epitope description:S85, Q86, K87, N88, V89, A90, Q95, T96, N97, Y99, Y102, C136, E137, G138, Y141
antigen name:Ribonuclease pancreatic
host organism:Camelus dromedarius
antibody name:affinity matured cAb-RN05 clone a
|
| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
17888451 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1019700 |
| landingPage: |
http://www.iedb.org/assay/1796308 |
| type: |
Literature
|
| publicationVenue: |
J Mol Biol
|
| dates: |
2007
|
| study type: | b cell assays |
| subject species: | Bos taurus |
| fullName: |
Akiko Koide
Valentina Tereshko
Serdar Uysal
Katrina Margalef
Anthony A Kossiakoff
Shohei Koide
|
| method: |
binding assay
|
| name: |
Exploring the capacity of minimalist protein interfaces: interface energetics and affinity maturation to picomolar KD of a single-domain antibody with a flat paratope.
|
| description: |
The epitope residues are calculated from PDB structure at 4Å
atomic distance from the antibody.
The epitope specific single domain VHH affinity matured cAb-RN05 (clone a) was analyzed for binding using a yeast surface display system and measured by flow cytometry. Alanine scanning mutagenesis of clone a identified five residues that contributed to enhanced binding in the affinity-matured antibody.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
