Monoclonal antibody 76F distinguishes IA-2 from IA-2beta and overlaps an autoantibody epitope. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Monoclonal antibody 76F distinguishes IA-2 from IA-2beta and overlaps an autoantibody epitope.

Dataset

identifier:	1630872
description:	epitope description:FEYQD antigen name:Receptor-type tyrosine-protein phosphatase-like N host organism:Mus musculus BALB/c antibody name:76F
aggregation:	instance of dataset
availability:	available
primaryPublications:	16503116

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1007458
landingPage:	http://www.iedb.org/assay/1630872

Publication

type:	Literature
publicationVenue:	J Autoimmun
dates:	2006

Dimension

study type:	b cell assays
subject species:	Homo sapiens

Person

fullName:	Sandra Piquer Lionel Valera Vito Lampasona Bé né dicte Jardin-Watelet Sté phanie Roche Claude Granier Francoise Roquet Michael R Christie Tiziana Giordano Maria-Luisa Malosio Ezio Bonifacio Daniel Laune

Data Type

method:	western blot

Study

name:	Monoclonal antibody 76F distinguishes IA-2 from IA-2beta and overlaps an autoantibody epitope.
description:	The epitope-specific monoclonal antibody was shown to bind in Western blots to rat and human islets, the &betaTC3 cell line and pituitary, but not to neuroblastoma cell lines, or the 3T3 cell line. This differential binding is consistent with the tissue and cell expression of IA-2 (the source protein of the epitope) and IA-2beta. Similar results were obtained in immunoprecipitation assays that showed that the mAb binds human, rat and mouse IA-2, but did not bind IA-2beta of any species.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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