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Title: Distinct antigenic features of linear epitopes at the N-terminus and C-terminus of 65 kDa glutamic acid decarboxylase (GAD65): implications for autoantigen modification during pathogenesis.
identifier: 1620871
description:
epitope description:DFLIEEIERLGQDL
antigen name:Glutamate decarboxylase 2
host organism:Oryctolagus cuniculus New Zealand White
antibody name:C-pc
aggregation:
instance of dataset
availability:
available
primaryPublications: 12296864
authorizations:
registration not required
accessURL: http://www.iedb.org/reference/1014164
landingPage: http://www.iedb.org/assay/1620871
type:
Literature
publicationVenue:
Clin Exp Immunol
dates:
2002
study type: b cell assays
subject species: Homo sapiens
fullName:
T A M A Al-Bukhari
P M Radford
G Bouras
C Davenport
S M Trigwell
G-F Bottazzo
M Lai
H L Schwartz
P J Tighe
I
method:
phage display
name:
Distinct antigenic features of linear epitopes at the N-terminus and C-terminus of 65 kDa glutamic acid decarboxylase (GAD65): implications for autoantigen modification during pathogenesis.
description:
The epitope sequence is also found in GAD67 (residues 581-594).
The rabbit anti-epitope antiserum selected the peptide from a T7 phage library expressing 9-mer peptide inserts constrained by a difulfide bridge after immunopanning. The peptide had homology to GAD65 residues 582-585. The suggested consensus epitope for the C-pc antibody is GQDL, spanning residues 582-585 of GAD65. Analysis of the immunoselected peptides suggests that the C-pc polyclonal antibodies recognize two distinct sites within the epitope sequence.

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