Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1014290 |
| landingPage: |
http://www.iedb.org/assay/1632514 |
| type: |
Literature
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| publicationVenue: |
J Autoimmun
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| dates: |
2006
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| study type: | b cell assays |
| subject species: | Homo sapiens |
| fullName: |
Karen H O'Connor
J Paul Banga
Connie Darmanin
Ossama El-Kabbani
Ian R Mackay
Merrill J Rowley
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| method: |
immunoprecipitation
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| name: |
Characterisation of an autoreactive conformational epitope on GAD65 recognised by the human monoclonal antibody b78 using a combination of phage display, in vitro mutagenesis and molecular modelling.
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| description: |
This epitope was defined through analysis of mutated glutamate decarboxylase 2 binding to a human monoclonal antibody.
The epitope specific monoclonal antibody bound glutamate decarboxylase 2. Amino acids 524-526, 572-573, and 498-500 were shown to be important for binding by alanine mutagenesis. Binding of gluatmine decarboxylase 2 to GAD1 and GAD6 monoclonal antibodies was also affected by mutating 572-573. This indicates that this mutation has structural effects rather than direct contact with the monoclonal antibody.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
