Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1014377 |
| landingPage: |
http://www.iedb.org/assay/1639246 |
| type: |
Literature
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| publicationVenue: |
Viral Immunol
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| dates: |
2000
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| study type: | b cell assays |
| subject species: | La Crosse virus |
| fullName: |
L L Cheng
K T Schultz
T M Yuill
B A Israel
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| method: |
immuno staining
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| name: |
Identification and localization of conserved antigenic epitopes on the G2 proteins of California serogroup Bunyaviruses.
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| description: |
The epitope was deduced.
Competitive binding ELISA was used to define antigenic regions. Indirect fluorescent assay was used to demonstrate that replacement of the epitope with the sequence from bunyamwera virus G2 caused a complete loss of binding, indicating it contributes to a discontinuous epitope. Replacement of other regions (29-34 and 50-58) also reduced binding, indicating they also contribute to the discontinuous epitope. G2 protein was expressed in recombinant vaccinia-T7 virus-infected HeLa cells.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
