Immunological Data Discovery Index
Immunological Data Discovery Index
| identifier: | 1653849 |
| description: |
epitope description:N64, H65, T66, P67, P68, G69, S70, Y71, T118, H119, I120, S121, V122, Q266, G267, A268, R269, S270, V271, R272, A273, V274, N275, E276, E277, S278, Q279, P280, E281
antigen name:Shiga toxin 1 subunit A
host organism:Mus musculus BALB/c
antibody name:11E10
|
| aggregation: |
instance of dataset
|
| availability: |
available
|
| primaryPublications: |
19433543 |
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1015927 |
| landingPage: |
http://www.iedb.org/assay/1653849 |
| type: |
Literature
|
| publicationVenue: |
Infect Immun
|
| dates: |
2009
|
| study type: | b cell assays |
| subject species: | Escherichia coli |
| fullName: |
Michael J Smith
Angela R Melton-Celsa
James F Sinclair
Humberto M Carvalho
Cory M Robinson
Alison D O'Brien
|
| method: |
western blot
|
| name: |
Monoclonal antibody 11E10, which neutralizes shiga toxin type 2 (Stx2), recognizes three regions on the Stx2 A subunit, blocks the enzymatic action of the toxin in vitro, and alters the overall cellular distribution of the toxin.
|
| description: |
The epitope-specific mAb bound to a chimeric molecule of Shiga toxin 1 that contained the discontinuous epitope from Shiga toxin 2: residues 42-49 (region A), 96-100 (B), and 244-259 (C). The mAb bound to chimeras replaced only with region A, A+B, and A+C, but not B+C.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
