Structure of rotavirus outer-layer protein VP7 bound with a neutralizing Fab. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Structure of rotavirus outer-layer protein VP7 bound with a neutralizing Fab.

Dataset

identifier:	1658277
description:	epitope description:N96, K194, D211, T212, A213, T214, E217, T220 antigen name:Outer capsid glycoprotein VP7 host organism:Mus musculus antibody name:4F8
aggregation:	instance of dataset
availability:	available
primaryPublications:	19520960

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1015107
landingPage:	http://www.iedb.org/assay/1658277

Publication

type:	Literature
publicationVenue:	Science
dates:	2009

Dimension

study type:	b cell assays
subject species:	Rhesus rotavirus

Person

fullName:	Scott T Aoki Ethan C Settembre Shane D Trask Harry B Greenberg Stephen C Harrison Philip R Dormitzer

Data Type

method:	x-ray crystallography

Study

name:	Structure of rotavirus outer-layer protein VP7 bound with a neutralizing Fab.
description:	The epitope residues were calculated based on the PDB structure [PDB: 3FMG] as the antigen residues interacting with the antibody at 4 Å atomic distance. This epitope is located on one subunit of the antigen, which is a trimer. This epitope is a part of the larger epitope located on two subunits of the antigen trimer. The epitope-specific mAb 4F8 binds only Ca2+ bound, trimeric VP7. Three Fab fragments bind each trimer. The asymmetric unit contains one monomer (subunit) of VP7 and one Fab molecule. The Fab binds across the outer surface of the inter-subunit contact. Therefore, the whole epitope is located on two subunits, meaning that the epitope derived from the PDB structure is a partial epitope.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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