Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1013853 |
| landingPage: |
http://www.iedb.org/assay/1660773 |
| type: |
Literature
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| publicationVenue: |
J Allergy Clin Immunol
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| dates: |
2009
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| study type: | b cell assays |
| subject species: | Mammalia |
| fullName: |
Scott P Commins
Shama M Satinover
Jacob Hosen
Jonathan Mozena
Larry Borish
Barrett D Lewis
Judith A Woodfolk
Thomas A E Platts-Mills
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| method: |
ELISA
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| name: |
Delayed anaphylaxis, angioedema, or urticaria after consumption of red meat in patients with IgE antibodies specific for galactose-alpha-1,3-galactose.
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| description: |
alpha-gal was coupled to sepharose via a linker (Galalpha1-3Galbeta-OCH2CH2CH2NH-sepharose) and used in direct ELISA and pre-adsorption experiments. All donors had alpha-gal serum IgEs, and there is a significant correlation between IgE antibodies to alpha-gal and IgE antibodies to beef. Reactivity to beef, pork, cat and dog extracts was significantly reduced by pre-adsorption of the sera with the epitope, while no effect was seen on the reactivity to non-mammalian extracts.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
