Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
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| accessURL: |
http://www.iedb.org/reference/1012698 |
| landingPage: |
http://www.iedb.org/assay/1662778 |
| type: |
Literature
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| publicationVenue: |
Immunology
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| dates: |
1978
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| study type: | b cell assays |
| subject species: |
| fullName: |
W R Thomas
M C Watkins
S S Jouhal
G L Asherson
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| method: |
in vivo skin test
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| name: |
Induction and modification of anti-TNP reaginic and IgG antibody responses by reactive trinitrophenyl derivatives.
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| description: |
Painting with 5 mg picryl chloride elicited anti-TNP IgE (reagin) antibodies that were maximal after the third painting, whereas feeding picryl chloride, or injecting picryl sulphonic acid did not. IgE titers were measured by passive cutaneous anaphylaxis reactions in rat skin 24 h after sensitization. In separate experiments, IgE antibody-forming cells were measured by heterologous adoptive cutaneous anaphylaxis (HACA) by injecting immune spleen or lymph node cells into the abdomen of rats and 24 h later challenging with TNP-BSA and Evans blue dye and measuring the response at 45 min. Painting with 5 mg picryl chloride elicited IgE antibody-forming cells first in the lymph nodes at day 6 and maximal in the spleen and lymph nodes at day 23. Feeding picryl chloride or injecting picryl sulphonic acid did elicit a response. The IgE response was T-cell dependent as nude mice showed no antibody response to picryl chloride. Adult thymectomy did not affect the response.
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| name: |
iedb
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| homePage: |
http://www.iedb.org |
