Availability of dinitrophenylated lipid haptens for specific antibody binding depends on the physical properties of host bilayer membranes.

Title:

Availability of dinitrophenylated lipid haptens for specific antibody binding depends on the physical properties of host bilayer membranes.

identifier:	1663816
description:	epitope description:aminoxyl host organism:Mus musculus antibody name:AN01
aggregation:	instance of dataset
availability:	available
primaryPublications:	7076676

study type:	b cell assays
subject species:

fullName:	K Balakrishnan S Q Mehdi H M McConnell

method:	radio immuno assay (RIA)

name:	Availability of dinitrophenylated lipid haptens for specific antibody binding depends on the physical properties of host bilayer membranes.
description:	Anti-nitroxide IgG1 monoclonal antibody bound to dipalmitoylphophatidylcholine liposomes containing nitroxide lipid hapten II with an apparent binding constant of 1.5 x 10⁸ M^-1 at 0 C or 37 C, temperatures below the lipid transition temperature. Anti-nitroxide IgG1 mAb bound to nitroxide lipid hapten II liposomes containing dimyristoylphophatidylcholine (transition temperature = 22 C) both at 0 C (solid membranes) or at 37 C (fluid membranes) with similar affinity. The structure of nitroxide lipid hapten II is found in the reference cited: Balakrishnan et al. (1982) J Biol Chem 257: 6427-6433 [PMID: 6281271].

name:	iedb
homePage:	http://www.iedb.org

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