Fusion loop peptide of the West Nile virus envelope protein is essential for pathogenesis and is recognized by a therapeutic cross-reactive human monoclonal antibody.

Title:

Fusion loop peptide of the West Nile virus envelope protein is essential for pathogenesis and is recognized by a therapeutic cross-reactive human monoclonal antibody.

Dataset

identifier:	1665347
description:	epitope description:W101, G104, G106, T484 antigen name:Genome polyprotein host organism:Homo sapiens antibody name:mAb11
aggregation:	instance of dataset
availability:	available
primaryPublications:	19535627

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1014889
landingPage:	http://www.iedb.org/assay/1665347

Publication

type:	Literature
publicationVenue:	J Immunol
dates:	2009

Dimension

study type:	b cell assays
subject species:	West Nile virus strain 2741

Person

fullName:	Hameeda Sultana Harald G Foellmer Girish Neelakanta Theodore Oliphant Michael Engle Michel Ledizet Manoj N Krishnan Nathalie Bonafé Karen G Anthony Wayne A Marasco Paul Kaplan Ruth R Montgomery Michael S Diamond Raymond A Koski Erol Fikrig

Data Type

method:	western blot

Study

name:	Fusion loop peptide of the West Nile virus envelope protein is essential for pathogenesis and is recognized by a therapeutic cross-reactive human monoclonal antibody.
description:	Recognition of WT Env protein by the epitope-specific mAb occurred only under non-denaturing conditions, confirming the conformational nature of the epitope. Recognition of recombinant truncated Env including only domains I and II in a yeast-display system was abrogated for mutant proteins with substitutions at residues W101, G104 and G106. Recognition of WT Env was detected also in ELISA.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

Feedback?

If you are having problems using our tools, or if you would just like to send us some feedback, please post your questions on Feedback.

Related Publications

Feedback?