High-resolution autoreactive epitope mapping and structural modeling of the 65 kDa form of human glutamic acid decarboxylase.

Title:

High-resolution autoreactive epitope mapping and structural modeling of the 65 kDa form of human glutamic acid decarboxylase.

identifier:	1665691
description:	epitope description:R536, Y540 antigen name:Glutamate decarboxylase 2 host organism:Homo sapiens antibody name:M8 (MICA-8), M9 (MICA-9)
aggregation:	instance of dataset
availability:	available
primaryPublications:	10222205

study type:	b cell assays
subject species:	Homo sapiens

fullName:	H L Schwartz J M Chandonia S F Kash J Kanaani E Tunnell A Domingo F E Cohen J P Banga A M Madec W Richter S Baekkeskov

method:	immunoprecipitation

name:	High-resolution autoreactive epitope mapping and structural modeling of the 65 kDa form of human glutamic acid decarboxylase.
description:	The epitope recognized by the human mAbs was deduced based on reactivity with GAD65/67 chimeric proteins. Swapping residues 532-540 of GAD65 with those from GAD67 abolished reactivity with M8 and M9. Single amino acid substitution of R536L or Y540S abolished reactivity with M8 and M9, indicating they are critical residues for these mAbs. Single amino acid substitution H470Q also reduces M8 and M9 reactivity, indicating that other regions affect the epitope.

name:	iedb
homePage:	http://www.iedb.org

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