Immunological Data Discovery Index
Immunological Data Discovery Index
| authorizations: |
registration not required
|
| accessURL: |
http://www.iedb.org/reference/1005421 |
| landingPage: |
http://www.iedb.org/assay/1678656 |
| type: |
Literature
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| publicationVenue: |
Protein Sci
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| dates: |
2003
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| study type: | b cell assays |
| subject species: |
| fullName: |
Leo C James
Dan S Tawfik
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| method: |
ELISA
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| name: |
The specificity of cross-reactivity: promiscuous antibody binding involves specific hydrogen bonds rather than nonspecific hydrophobic stickiness.
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| description: |
Competition ELISA with soluble DNP for binding to DNP-biotin was performed. A similar result (Kd =40 nM) was obtained with DNP-lysine, which is the residue through which the DNP is linked to the immunizing carrier, while low affinity (Kd 10000 nM) was obtained to DNP-Pro. Fluorescence quenching assays with DNP-Asn and DNP-Ser gave a Kd of 700 and 20 nM, respectively.
|
| name: |
iedb
|
| homePage: |
http://www.iedb.org |
