Molecular basis of allergen cross-reactivity: non-specific lipid transfer proteins from wheat flour and peach fruit as models.

Title:

Molecular basis of allergen cross-reactivity: non-specific lipid transfer proteins from wheat flour and peach fruit as models.

identifier:	1687841
description:	epitope description:SPQRMQAFNYS host organism:Homo sapiens
aggregation:	instance of dataset
availability:	available
primaryPublications:	19846220

study type:	b cell assays
subject species:

fullName:	Leticia Tordesillas Luis F Pacios Arancha Palacin Santiago Quirce Alicia Armentia Domingo Barber Gabriel Salcedo Araceli Diaz-Perales

method:	ELISA

name:	Molecular basis of allergen cross-reactivity: non-specific lipid transfer proteins from wheat flour and peach fruit as models.
description:	The epitope is a mimotope of discontinuous IgE binding epitope on Tri a 14 including residues L34, H35, N36, R39, S40, S42, D43, P70, L77, P78, Y79, and T80. The epitope sequence was selected from a random peptide phage-displayed library by pooled serum IgE from patients with baker's asthma caused by wheat flour (Tri a 14) and allergy to peach fruit (Pru p 3) and eluted with Tri a 14. Reactivity with patient IgE was confirmed by ELISA. The selected phage sequences were mapped to a consensus sequence on the surface of Tri a 14 corresponding to residues L34, H35, N36, R39, S40, S42, D43, P70, L77, P78, Y79, and T80. Comparing sequences of peptides eluted with Tri a 14 after elution with Pru p 3, the authors deduced a final epitope corresponding to residues H35, N36, R39, S40, S42, D43, G74, V75, L77, P78, Y79, and T80.

name:	iedb
homePage:	http://www.iedb.org

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