Mapping of SLE-specific Sm B cell epitopes using murine monoclonal antibodies. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Mapping of SLE-specific Sm B cell epitopes using murine monoclonal antibodies.

Dataset

identifier:	1691743
description:	epitope description:PKVKSKKREAVAGRGRGRGRGRGRGRGRGRGGPRR antigen name:Small nuclear ribonucleoprotein Sm D1 host organism:Mus musculus MLR/lpr antibody name:7.13
aggregation:	instance of dataset
availability:	available
primaryPublications:	9185874

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1007321
landingPage:	http://www.iedb.org/assay/1691743

Publication

type:	Literature
publicationVenue:	J Autoimmun
dates:	1997

Dimension

study type:	b cell assays
subject species:	Homo sapiens

Person

fullName:	G J Pruijn F Schoute J P Thijssen R J Smeenk W J van Venrooij

Data Type

method:	immunoprecipitation

Study

name:	Mapping of SLE-specific Sm B cell epitopes using murine monoclonal antibodies.
description:	The epitope was deduced by immuno precipitation of in vitro translated mutants by analogy to the epitope for mAb 7.13. The results of the immunoprecipitation show that the epitope recognized by mAb 7.13 was located in the carboxy-terminal region. A deletion of the 11 carboxy-terminal amino acids (108-119) almost entirely abolished recognition, whereas the deletion of amino acids 85-119 completely abolished reactivity of mAb 7.13. Mapping of another Sm-D-specific mAb ANA127 (IgA) gave similar results (data not shown).

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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