Monoclonal antibodies to the West Nile virus NS5 protein map to linear and conformational epitopes in the methyltransferase and polymerase domains.

Title:

Monoclonal antibodies to the West Nile virus NS5 protein map to linear and conformational epitopes in the methyltransferase and polymerase domains.

Dataset

identifier:	1696860
description:	epitope description:KHARKERNITGGH antigen name:Genome polyprotein host organism:Mus musculus BALB/c antibody name:5H1
aggregation:	instance of dataset
availability:	available
primaryPublications:	19710254

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1017769
landingPage:	http://www.iedb.org/assay/1696860

Publication

type:	Literature
publicationVenue:	J Gen Virol
dates:	2009

Dimension

study type:	b cell assays
subject species:	Kunjin virus

Person

fullName:	Roy A Hall Si En Tan Barbara Selisko Rachael Slade Jody Hobson-Peters Bruno Canard Megan Hughes Jason Y Leung Ezequiel Balmori-Melian Sonja Hall-Mendelin Kim B Pham David C Clark Natalie A Prow Alexander A Khromykh

Data Type

method:	ELISA

Study

name:	Monoclonal antibodies to the West Nile virus NS5 protein map to linear and conformational epitopes in the methyltransferase and polymerase domains.
description:	Truncation experiments show that this is the minimal epitope for the mAb in ELISA and microsphere immunoassay. Amino acid substitutions at residues R7 and I9 dramatically reduced binding affinity by the mAb, indicating these residues as critical for mAb binding site.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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