Epitope mapping and serodiagnosis using Ata- and (Lys)7 peptides. | ImmuneData Discovery Index

Immunological Data Discovery Index

Immunological Data Discovery Index

Title:

Epitope mapping and serodiagnosis using Ata- and (Lys)7 peptides.

Dataset

identifier:	1699811
description:	epitope description:LEDPASRDLVVN + SAMA(L1) antigen name:Capsid protein host organism:Mus musculus BALB/c antibody name:anti-HBe4
aggregation:	instance of dataset
availability:	available
primaryPublications:	9528663

Access

authorizations:	registration not required
accessURL:	http://www.iedb.org/reference/1018132
landingPage:	http://www.iedb.org/assay/1699811

Publication

type:	Literature
publicationVenue:	Biochim Biophys Acta
dates:	1998

Dimension

study type:	b cell assays
subject species:	Hepatitis B virus

Person

fullName:	E E Loomans L J Nijholt A J Cremers W P Paulij W J Schielen

Data Type

method:	antigen inhibition

Study

name:	Epitope mapping and serodiagnosis using Ata- and (Lys)7 peptides.
description:	The epitope of HBV non-particulate e-antigen was N-terminally extended by an acetyl-thio-acetyl group. The minimal epitope recognized by mAb anti-HBe4 was determined by direct binding or by competition ELISA with C-terminally truncated peptides. The 12-mer epitope was the minimal truncation of the 17-mer peptide (LEDPASRDLVVNYVNTN) that inhibited binding. The 15-mer (LEDPASRDLVVNYVN) was required for direct binding. The peptides were N-terminally extended with an acetyl-thio-acetyl group, as no signal was detected with unmodified peptides.

Data Repository

name:	iedb
homePage:	http://www.iedb.org

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