Delineation of the discontinuous-conformational epitope of a monoclonal antibody displaying full in vitro and in vivo thyrotropin activity.

Title:

Delineation of the discontinuous-conformational epitope of a monoclonal antibody displaying full in vitro and in vivo thyrotropin activity.

identifier:	1713258
description:	epitope description:T62, K64, R86, Y88, R115, F136, G138, F140 antigen name:Thyrotropin receptor host organism:Mus musculus antibody name:1H7
aggregation:	instance of dataset
availability:	available
primaryPublications:	15319453

study type:	b cell assays
subject species:	Homo sapiens

fullName:	Sabine Costagliola Marco Bonomi Nils G Morgenthaler Joost Van Durme Valé rie Panneels Samuel Refetoff Gilbert Vassart

method:	flow cytometry

name:	Delineation of the discontinuous-conformational epitope of a monoclonal antibody displaying full in vitro and in vivo thyrotropin activity.
description:	The epitope residues recognized by mAb 1H7 were deduced based on reactivity with human TSHR mutated at one or more residues or chimeras of receptors for TSHR and LH/CG. The mAb recognizes region 1-281 of hTSHR, as well as TSHR from mouse, rat, cat, dog, and sheep. Mutation of single residues T56, K58, R80, Y82, or R109 abolished binding, indicating these are critical residues. In addition, the triple mutation of F130, G132, and F134 reduced binding, indicating these residues contribute to the antibody binding site. MAb 1H7 inhibits the binding of bovine TSH to the TSH receptor in a dose-dependent manner.

name:	iedb
homePage:	http://www.iedb.org

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